WDR85 (DPH7) (NM_138778) Human Recombinant Protein

• Background:

  Diphthamide is a post-translationally modified histidine residue present in elongation factor 2, and is the target of diphtheria toxin. This gene encodes a protein that contains a WD-40 domain, and is thought to be involved in diphthamide biosynthesis. A similar protein in yeast functions as a methylesterase, converting methylated diphthine to diphthine, which can then undergo amidation to produce diphthamide. [provided by RefSeq, Oct 2016]

• Synonyms:

  C9orf112; RRT2; WDR85

• Gene ID:

  92715

• UniProt:

  Q9BTV6

• Accession Number mRNA:

  NM_138778

• Chromosomal Location:

  9q34.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  50.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  DPH7

• Species:

  Human

• Protein ID:

  NP_620133

• Overview:

  Recombinant protein of human WD repeat domain 85 (WDR85)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/92715

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9BTV6

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_138778

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_620133