Factor XIIIa (F13A1) (NM_000129) Human Recombinant Protein

• Background:

  This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]

• Synonyms:

  F13A

• Gene ID:

  2162

• UniProt:

  P00488

• Accession Number mRNA:

  NM_000129

• Chromosomal Location:

  6p25.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Complement and coagulation cascades

• Field of Research:

  Complement and coagulation cascades

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Secreted Protein

• Molecular Weight:

  79.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  F13A1

• Species:

  Human

• Protein ID:

  NP_000120

• Overview:

  Recombinant protein of human coagulation factor XIII, A1 polypeptide (F13A1)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2162

• Uniprot URL:

  https://www.uniprot.org/uniprot/P00488

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000129

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000120