Pyruvate Dehydrogenase E2 (DLAT) (NM_001931) Human Recombinant Protein

• Background:

  This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC) . PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC) . In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]

• Synonyms:

  DLTA; E2; PBC; PDC-E2; PDCE2

• Gene ID:

  1737

• UniProt:

  P10515, Q86YI5

• Accession Number mRNA:

  NM_001931

• Chromosomal Location:

  11q23.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism

• Field of Research:

  Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  59.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  DLAT

• Species:

  Human

• Protein ID:

  NP_001922

• Overview:

  Recombinant protein of human dihydrolipoamide S-acetyltransferase (DLAT)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/1737

• Uniprot URL:

  https://www.uniprot.org/uniprot/P10515

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001931

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001922