YTHDF1 (NM_017798) Human Recombinant Protein

• Background:

  Specifically recognizes and binds N6-methyladenosine (m6A) -containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451) . M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625) . Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440) . Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory (By similarity) . Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation (By similarity) . Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (By similarity) . Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells (By similarity) . In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  C20orf21

• Gene ID:

  54915

• UniProt:

  Q9BYJ9

• Accession Number mRNA:

  NM_017798

• Chromosomal Location:

  20q13.33

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  60.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  YTHDF1

• Species:

  Human

• Protein ID:

  NP_060268

• Overview:

  Recombinant protein of human YTH domain family, member 1 (YTHDF1)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/54915

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9BYJ9

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_017798

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_060268