C20orf77 (RPRD1B) (NM_021215) Human Recombinant Protein

• Background:

  Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  C20orf77; CREPT; dJ1057B20.2; K-H; Kub5-Hera; NET60

• Gene ID:

  58490

• UniProt:

  Q9NQG5

• Accession Number mRNA:

  NM_021215

• Chromosomal Location:

  20q11.23

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  36.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  RPRD1B

• Species:

  Human

• Protein ID:

  NP_067038

• Overview:

  Recombinant protein of human regulation of nuclear pre-mRNA domain containing 1B (RPRD1B)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/58490

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9NQG5

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_021215

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_067038