Deleted in azoospermia 4 (DAZ4) (NM_020420) Human Recombinant Protein

• Background:

  This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF) . Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains two copies of the 10.8 kb repeat. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2011]

• Synonyms:

  PDP1680; pDP1681

• Gene ID:

  57135

• UniProt:

  Q86SG3, Q658T2, Q86SG3-2

• Accession Number mRNA:

  NM_020420

• Chromosomal Location:

  Yq11.23

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  43.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  DAZ4

• Species:

  Human

• Protein ID:

  NP_065153

• Overview:

  Purified recombinant protein of Homo sapiens deleted in azoospermia 4 (DAZ4), transcript variant 2

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/57135

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q86SG3

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_020420

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_065153