RAP1GAP (NM_002885) Human Recombinant Protein

• Background:

  This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011]

• Synonyms:

  RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP

• Gene ID:

  5909

• UniProt:

  P47736, A0A024RAB5

• Accession Number mRNA:

  NM_002885

• Chromosomal Location:

  1p36.12

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  73.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  RAP1GAP

• Species:

  Human

• Protein ID:

  NP_002876

• Overview:

  Recombinant protein of human RAP1 GTPase activating protein (RAP1GAP), transcript variant 3

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/5909

• Uniprot URL:

  https://www.uniprot.org/uniprot/P47736

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_002885

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_002876