ACTN2 (NM_001103) Human Recombinant Protein

• Background:

  Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

• Synonyms:

  CMD1AA; CMH23; MPD6; MYOCOZ

• Gene ID:

  88

• UniProt:

  P35609

• Accession Number mRNA:

  NM_001103

• Chromosomal Location:

  1q43

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction

• Field of Research:

  Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  103.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ACTN2

• Species:

  Human

• Protein ID:

  NP_001094

• Overview:

  Recombinant protein of human actinin, alpha 2 (ACTN2)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/88

• Uniprot URL:

  https://www.uniprot.org/uniprot/P35609

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001103

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001094