Natriuretic Peptide Receptor A (NPR1) (NM_000906) Human Recombinant Protein

• Background:

  Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]) . The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961) . Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively) .[supplied by OMIM, May 2009]

• Synonyms:

  ANPa; ANPRA; GUC2A; GUCY2A; NPRA

• Gene ID:

  4881

• UniProt:

  P16066, A0A140VJE6

• Accession Number mRNA:

  NM_000906

• Chromosomal Location:

  1q21.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Purine metabolism, Vascular smooth muscle contraction

• Field of Research:

  Purine metabolism, Vascular smooth muscle contraction

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Protein Kinase

• Molecular Weight:

  118.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NPR1

• Species:

  Human

• Protein ID:

  NP_000897

• Overview:

  Recombinant protein of human natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) (NPR1)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/4881

• Uniprot URL:

  https://www.uniprot.org/uniprot/P16066

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000906

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000897