ASK1 (MAP3K5) (NM_005923) Human Recombinant Protein

• Background:

  Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK) . MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK) /stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

• Synonyms:

  ASK1; MAPKKK5; MEKK5

• Gene ID:

  4217

• UniProt:

  Q99683

• Accession Number mRNA:

  NM_005923

• Chromosomal Location:

  6q23.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway, Neurotrophin signaling pathway

• Field of Research:

  Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway, Neurotrophin signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Protein Kinase

• Molecular Weight:

  154.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MAP3K5

• Species:

  Human

• Protein ID:

  NP_005914

• Overview:

  Recombinant protein of human mitogen-activated protein kinase kinase kinase 5 (MAP3K5)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/4217

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q99683

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_005923

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_005914