HLAB (HLA-B) (NM_005514) Human Recombinant Protein

• Background:

  HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

• Synonyms:

  AS; B-4901; HLAB; SPDA1

• Gene ID:

  3106

• UniProt:

  P01889, P03989, E5FQ95, P30480, P30461

• Accession Number mRNA:

  NM_005514

• Chromosomal Location:

  6p21.33

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis

• Field of Research:

  Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Transmembrane

• Molecular Weight:

  37.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  HLA-B

• Species:

  Human

• Protein ID:

  NP_005505

• Overview:

  Recombinant protein of human major histocompatibility complex, class I, B (HLA-B)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/3106

• Uniprot URL:

  https://www.uniprot.org/uniprot/P01889

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_005514

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_005505