Factor VII (F7) (NM_019616) Human Recombinant Protein

• Background:

  This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]

• Synonyms:

  SPCA

• Gene ID:

  2155

• UniProt:

  P08709

• Accession Number mRNA:

  NM_019616

• Chromosomal Location:

  13q34

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Complement and coagulation cascades

• Field of Research:

  Complement and coagulation cascades

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Protease

• Molecular Weight:

  28 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  F7

• Species:

  Human

• Protein ID:

  NP_062562

• Overview:

  Recombinant protein of human coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant 2

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2155

• Uniprot URL:

  https://www.uniprot.org/uniprot/P08709

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_019616

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_062562