Phospholipase C epsilon 1 (PLCE1) (NM_016341) Human Recombinant Protein

• Background:

  This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) . These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]

• Synonyms:

  NPHS3; PLCE; PPLC

• Gene ID:

  51196

• UniProt:

  Q9P212

• Accession Number mRNA:

  NM_016341

• Chromosomal Location:

  10q23.33

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system

• Field of Research:

  Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  258.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PLCE1

• Species:

  Human

• Protein ID:

  NP_057425

• Overview:

  Purified recombinant protein of Homo sapiens phospholipase C, epsilon 1 (PLCE1)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/51196

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9P212

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016341

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_057425