MID1 (NM_001098624) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]

• Synonyms:

  BBBG1; FXY; GBBB1; MIDIN; OGS1; OS; OSX; RNF59; TRIM18; XPRF; ZNFXY

• Gene ID:

  4281

• UniProt:

  O15344, A0A024RBV4

• Accession Number mRNA:

  NM_001098624

• Chromosomal Location:

  Xp22.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Ubiquitin mediated proteolysis

• Field of Research:

  Ubiquitin mediated proteolysis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  75.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MID1

• Species:

  Human

• Protein ID:

  NP_001092094

• Overview:

  Recombinant protein of human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 4

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/4281

• Uniprot URL:

  https://www.uniprot.org/uniprot/O15344

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001098624

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001092094