STK23 (SRPK3) (NM_014370) Human Recombinant Protein

• Background:

  This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

• Synonyms:

  MSSK-1; MSSK1; STK23

• Gene ID:

  26576

• UniProt:

  Q9UPE1

• Accession Number mRNA:

  NM_014370

• Chromosomal Location:

  Xq28

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  SRPK3 activity verified in a biochemical assay: SRPK3 (SRSF protein kinase 3) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. SRPK3 is a serine/threonine kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. Varying concentrations of SRPK3 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Protein Kinase

• Molecular Weight:

  61.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SRPK3

• Species:

  Human

• Protein ID:

  NP_055185

• Overview:

  Recombinant protein of human SFRS protein kinase 3 (SRPK3)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/26576

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9UPE1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_014370

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_055185