CCBL1 (KYAT1) (NM_004059) Human Recombinant Protein

• Background:

  This gene encodes a cytosolic enzyme that is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism can form reactive metabolites leading to nephrotoxicity and neurotoxicity. Increased levels of this enzyme have been linked to schizophrenia. Multiple transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

• Synonyms:

  CCBL1; GTK; KAT1; KATI

• Gene ID:

  883

• UniProt:

  Q16773, A8K563

• Accession Number mRNA:

  NM_004059

• Chromosomal Location:

  9q34.11

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  The specific activity of KATI was determined by measuring the product Kynurenic acid formation from a conversion of Kynurenine. The reaction was carried out at 37? for 15min in the buffer containing 100 mM PBS, pH7.4, 2 mM a-oxoglutarate, 40µM PLP (pyridoxal 5'-phosphate), and 0.5 mM kynurenine as the substrate.

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  47.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  KYAT1

• Species:

  Human

• Protein ID:

  NP_004050

• Overview:

  Recombinant protein of human cysteine conjugate-beta lyase, cytoplasmic (CCBL1), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/883

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q16773

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_004059

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_004050