PDE12 (NM_177966) Human Recombinant Protein

• Background:

  Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. Degrades triphosphorylated 2-5A to produce AMP and ATP (PubMed:26055709) . Also cleaves 3',5'-phosphodiester bond of oligoadenylates (PubMed:21666256, PubMed:30389976, PubMed:26055709) . Plays a role as a negative regulator of the 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs) . Suppression of this enzyme increases cellular 2-5A levels and decreases viral replication in cultured small-airway epithelial cells and Hela cells (PubMed:26055709) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2 -PDE; 2-PDE; 2'-PDE

• Gene ID:

  201626

• UniProt:

  Q6L8Q7

• Accession Number mRNA:

  NM_177966

• Chromosomal Location:

  3p14.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  67.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PDE12

• Species:

  Human

• Protein ID:

  NP_808881

• Overview:

  Recombinant protein of human phosphodiesterase 12 (PDE12)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/201626

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q6L8Q7

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_177966

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_808881