PYK2 (PTK2B) (NM_173175) Human Recombinant Protein

• Background:

  This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

• Synonyms:

  CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK

• Gene ID:

  2185

• UniProt:

  Q14289, Q14289-2

• Accession Number mRNA:

  NM_173175

• Chromosomal Location:

  8p21.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Calcium signaling pathway, Chemokine signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity

• Field of Research:

  Calcium signaling pathway, Chemokine signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Protein Kinase

• Molecular Weight:

  111 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PTK2B

• Species:

  Human

• Protein ID:

  NP_775267

• Overview:

  Recombinant protein of human PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 4

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2185

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q14289

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_173175

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_775267