MEK3 (MAP2K3) (NM_002756) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

• Synonyms:

  MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2

• Gene ID:

  5606

• UniProt:

  P46734

• Accession Number mRNA:

  NM_002756

• Chromosomal Location:

  17p11.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Amyotrophic lateral sclerosis (ALS), Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway

• Field of Research:

  Amyotrophic lateral sclerosis (ALS), Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Protein Kinase, Transcription Factors

• Molecular Weight:

  36 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MAP2K3

• Species:

  Human

• Protein ID:

  NP_002747

• Overview:

  Recombinant protein of human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant A

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/5606

• Uniprot URL:

  https://www.uniprot.org/uniprot/P46734

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_002756

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_002747