Epac1 (RAPGEF3) (NM_001098532) Human Recombinant Protein

• Background:

  Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275

• Gene ID:

  10411

• UniProt:

  O95398, Q99777

• Accession Number mRNA:

  NM_001098532

• Chromosomal Location:

  12q13.11

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Leukocyte transendothelial migration, Long-term potentiation

• Field of Research:

  Leukocyte transendothelial migration, Long-term potentiation

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  99.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  RAPGEF3

• Species:

  Human

• Protein ID:

  NP_001092002

• Overview:

  Recombinant protein of human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 3

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/10411

• Uniprot URL:

  https://www.uniprot.org/uniprot/O95398

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001098532

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001092002