RASGRF1 (NM_002891) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]

• Synonyms:

  CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55; PP13187; ras-GRF1

• Gene ID:

  5923

• UniProt:

  Q13972, A8K270

• Accession Number mRNA:

  NM_002891

• Chromosomal Location:

  15q25.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Focal adhesion, MAPK signaling pathway

• Field of Research:

  Focal adhesion, MAPK signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  145.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  RASGRF1

• Species:

  Human

• Protein ID:

  NP_002882

• Overview:

  Recombinant protein of human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/5923

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q13972

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_002891

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_002882