POLR2J2 (POLR2J3) (NM_001097615) Human Recombinant Protein

• Background:

  This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD) . Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]

• Synonyms:

  POLR2J2; RPB11b1; RPB11b2

• Gene ID:

  548644

• UniProt:

  Q9GZM3, A0A0B4J2F8

• Accession Number mRNA:

  NM_001097615

• Chromosomal Location:

  7q22.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

• Field of Research:

  Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  12.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  POLR2J3

• Species:

  Human

• Protein ID:

  NP_001091084

• Overview:

  Recombinant protein of human polymerase (RNA) II (DNA directed) polypeptide J3 (POLR2J3)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/548644

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9GZM3

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001097615

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001091084