COASY (NM_025233) Human Recombinant Protein

• Background:

  Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5) . The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA) . Alternative splicing results in multiple isoforms. [provided by RefSeq, Apr 2014]

• Synonyms:

  DPCK; NBIA6; NBP; PCH12; pOV-2; PPAT; UKR1

• Gene ID:

  80347

• UniProt:

  Q13057

• Accession Number mRNA:

  NM_025233

• Chromosomal Location:

  17q21.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Metabolic pathways, Pantothenate and CoA biosynthesis

• Field of Research:

  Metabolic pathways, Pantothenate and CoA biosynthesis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  62.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  COASY

• Species:

  Human

• Protein ID:

  NP_079509

• Overview:

  Recombinant protein of human Coenzyme A synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/80347

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q13057

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_025233

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_079509