KIAA1967 (CCAR2) (NM_021174) Human Recombinant Protein

• Background:

  Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T) -rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation (PubMed:18235501, PubMed:18235502, PubMed:19131338, PubMed:19218236, PubMed:22446626, PubMed:23352644, PubMed:23398316) . Represses the ligand-dependent transcriptional activation function of ESR2 (PubMed:20074560) . Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1 (PubMed:24415752) . Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization (PubMed:21030595) . Positively regulates the beta-catenin pathway (canonical Wnt signaling pathway) and is required for MCC-mediated repression of the beta-catenin pathway (PubMed:24824780) . Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3 (PubMed:25661920) . Plays an important role in tumor suppression through p53/TP53 regulation; stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2 (PubMed:25732823) . Represses the transcriptional activator activity of BRCA1 (PubMed:20160719) . Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro (PubMed:25361978) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  DBC-1; DBC1; KIAA1967; NET35; p30 DBC; p30DBC

• Gene ID:

  57805

• UniProt:

  Q8N163

• Accession Number mRNA:

  NM_021174

• Chromosomal Location:

  8p21.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  102.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CCAR2

• Species:

  Human

• Protein ID:

  NP_066997

• Overview:

  Recombinant protein of human KIAA1967 (KIAA1967), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/57805

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8N163

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_021174

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_066997