RCC1 (NM_001269) Human Recombinant Protein

• Background:

  Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:1944575, PubMed:17435751, PubMed:20668449, PubMed:22215983, PubMed:11336674) . Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983) . Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983) . Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831) . Binds both to the nucleosomes and double-stranded DNA (PubMed:17435751, PubMed:18762580) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  CHC1; RCC1-I; SNHG3-RCC1

• Gene ID:

  1104

• UniProt:

  P18754, Q5T081

• Accession Number mRNA:

  NM_001269

• Chromosomal Location:

  1p35.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  44.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  RCC1

• Species:

  Human

• Protein ID:

  NP_001260

• Overview:

  Recombinant protein of human regulator of chromosome condensation 1 (RCC1), transcript variant 3

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/1104

• Uniprot URL:

  https://www.uniprot.org/uniprot/P18754

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001269

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001260