NARF (NM_012336) Human Recombinant Protein

• Background:

  Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq, Jul 2008]

• Synonyms:

  IOP2

• Gene ID:

  26502

• UniProt:

  Q9UHQ1

• Accession Number mRNA:

  NM_012336

• Chromosomal Location:

  17q25.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  51 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NARF

• Species:

  Human

• Protein ID:

  NP_036468

• Overview:

  Recombinant protein of human nuclear prelamin A recognition factor (NARF), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/26502

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9UHQ1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_012336

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_036468