Proteasome Activator Subunit 4 (PSME4) (NM_014614) Human Recombinant Protein

• Background:

  Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  PA200

• Gene ID:

  23198

• UniProt:

  Q14997

• Accession Number mRNA:

  NM_014614

• Chromosomal Location:

  2p16.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Proteasome

• Field of Research:

  Proteasome

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  211.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PSME4

• Species:

  Human

• Protein ID:

  NP_055429

• Overview:

  Recombinant protein of human proteasome (prosome, macropain) activator subunit 4 (PSME4)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/23198

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q14997

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_014614

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_055429