Aminoadipate aminotransferase (AADAT) (NM_016228) Human Recombinant Protein

• Background:

  This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

• Synonyms:

  KAT2; KATII; KYAT2

• Gene ID:

  51166

• UniProt:

  Q8N5Z0, Q4W5N8

• Accession Number mRNA:

  NM_016228

• Chromosomal Location:

  4q33

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Lysine biosynthesis, Lysine degradation, Metabolic pathways, Tryptophan metabolism

• Field of Research:

  Lysine biosynthesis, Lysine degradation, Metabolic pathways, Tryptophan metabolism

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  The specific activity of KATII was determined by measuring the product Kynurenic acid formation from a conversion of Kynurenine. The reaction was carried out at 37? for 15min in the buffer containing PBS, pH7.4, 2mM a-oxoglutarate, 40µM PLP (pyridoxal 5'-phosphate), and 0.5mM kynurenine as the substrate.

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  47.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  AADAT

• Species:

  Human

• Protein ID:

  NP_057312

• Overview:

  Recombinant protein of human aminoadipate aminotransferase (AADAT), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/51166

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8N5Z0

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016228

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_057312