Dematin (DMTN) (NM_001114135) Human Recombinant Protein

• Background:

  The protein encoded by this gene is an actin binding and bundling protein that plays a structural role in erythrocytes, by stabilizing and attaching the spectrin/actin cytoskeleton to the erythrocyte membrane in a phosphorylation-dependent manner. This protein contains a core domain in the N-terminus, and a headpiece domain in the C-terminus that binds F-actin. When purified from erythrocytes, this protein exists as a trimer composed of two 48 kDa polypeptides and a 52 kDa polypeptide. The different subunits arise from alternative splicing in the 3' coding region, where the headpiece domain is located. Disruption of this gene has been correlated with the autosomal dominant Marie Unna hereditary hypotrichosis disease, while loss of heterozygosity of this gene is thought to play a role in prostate cancer progression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]

• Synonyms:

  DMT; EPB49

• Gene ID:

  2039

• UniProt:

  Q08495

• Accession Number mRNA:

  NM_001114135

• Chromosomal Location:

  8p21.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  45.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  DMTN

• Species:

  Human

• Protein ID:

  NP_001107607

• Overview:

  Recombinant protein of human erythrocyte membrane protein band 4.9 (dematin) (EPB49), transcript variant 2

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2039

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q08495

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001114135

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001107607