MYL12B (NM_001144946) Human Recombinant Protein

• Background:

  The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]) .[supplied by OMIM, Mar 2008]

• Synonyms:

  MLC-B; MRLC2

• Gene ID:

  103910

• UniProt:

  O14950

• Accession Number mRNA:

  NM_001144946

• Chromosomal Location:

  18p11.31

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction

• Field of Research:

  Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  17.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MYL12B

• Species:

  Human

• Protein ID:

  NP_001138418

• Overview:

  Purified recombinant protein of Homo sapiens myosin, light chain 12B, regulatory (MYL12B), transcript variant 4

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/103910

• Uniprot URL:

  https://www.uniprot.org/uniprot/O14950

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001144946

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001138418