SAE1 (NM_016402) Human Recombinant Protein

• Background:

  Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]) .[supplied by OMIM, Mar 2010]

• Synonyms:

  AOS1; FLJ3091; HSPC140; SUA1; UBLE1A

• Gene ID:

  10055

• UniProt:

  Q9UBE0

• Accession Number mRNA:

  NM_016402

• Chromosomal Location:

  19q13.32

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Ubiquitin mediated proteolysis

• Field of Research:

  Ubiquitin mediated proteolysis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  38.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SAE1

• Species:

  Human

• Protein ID:

  NP_057486

• Overview:

  Recombinant protein of human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/10055

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9UBE0

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016402

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_057486