PDP1 (NM_001161781) Human Recombinant Protein

• Background:

  Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]

• Synonyms:

  PDH; PDP; PDPC; PPM2A; PPM2C

• Gene ID:

  54704

• UniProt:

  Q9P0J1, A0A024R9C0

• Accession Number mRNA:

  NM_001161781

• Chromosomal Location:

  8q22.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Phosphatase

• Molecular Weight:

  60.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PDP1

• Species:

  Human

• Protein ID:

  NP_001155253

• Overview:

  Recombinant protein of human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 4.

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/54704

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9P0J1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001161781

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001155253