C Kit (KIT) (NM_000222) Human Recombinant Protein

• Background:

  This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2020]

• Synonyms:

  C-Kit; CD117; MASTC; PBT; SCFR

• Gene ID:

  3815

• UniProt:

  P10721

• Accession Number mRNA:

  NM_000222

• Chromosomal Location:

  4q12

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer

• Field of Research:

  Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150mM NaCl, 10% glycerol

• Function:

  Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Protein Kinase, Stem cell - Pluripotency, Transmembrane

• Molecular Weight:

  33.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  KIT

• Species:

  Human

• Protein ID:

  NP_000213

• Overview:

  Purified recombinant protein of Human v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 1, Arg683-End, with N-terminal His tag, expressed in E.coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/3815

• Uniprot URL:

  https://www.uniprot.org/uniprot/P10721

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000222

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000213