Ube2i (BC037635) Mouse Recombinant Protein

• Background:

  Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Essential for nuclear architecture, chromosome segregation and embryonic viability. Necessary for sumoylation of FOXL2 and KAT5 (By similarity) . Sumoylates p53/TP53 at 'Lys-386'.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Mmubc9, UBC9, 5830467E05Rik

• Gene ID:

  22196

• UniProt:

  P63280

• Accession Number mRNA:

  BC037635

• Chromosomal Location:

  17 A3.3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  11.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ube2i

• Species:

  Mouse

• Overview:

  Purified recombinant protein of Mouse ubiquitin-conjugating enzyme E2I (cDNA clone MGC:46871 IMAGE:4951933), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/22196

• Uniprot URL:

  https://www.uniprot.org/uniprot/P63280

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/BC037635