Hspe1 (NM_008303) Mouse Recombinant Protein

• Background:

  Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  10kDa; Hsp10; mt-cpn10

• Gene ID:

  15528

• UniProt:

  Q64433, Q4KL76

• Accession Number mRNA:

  NM_008303

• Chromosomal Location:

  1 C1.2

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  11 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Hspe1

• Species:

  Mouse

• Protein ID:

  NP_032329

• Overview:

  Purified recombinant protein of Mouse heat shock protein 1 (chaperonin 10) (Hspe1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/15528

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q64433

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008303

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032329