Polr2f (NM_027231) Mouse Recombinant Protein

• Background:

  DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  1810060D16Rik; RPB6

• Gene ID:

  69833

• UniProt:

  P61219

• Accession Number mRNA:

  NM_027231

• Chromosomal Location:

  15 37.7 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  14.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Polr2f

• Species:

  Mouse

• Protein ID:

  NP_081507

• Overview:

  Purified recombinant protein of Mouse polymerase (RNA) II (DNA directed) polypeptide F (Polr2f), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/69833

• Uniprot URL:

  https://www.uniprot.org/uniprot/P61219

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_027231

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_081507