Cdc42 (NM_009861) Mouse Recombinant Protein

• Background:

  Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state (PubMed:24352656) . In its active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration (By similarity) . In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons (PubMed:25851601) . Facilitates filopodia formation upon DOCK11-activation (PubMed:22494997) . Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity) . Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI747189; AU018915

• Gene ID:

  12540

• UniProt:

  P60766

• Accession Number mRNA:

  NM_009861

• Chromosomal Location:

  4 69.83 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  21.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Cdc42

• Species:

  Mouse

• Protein ID:

  NP_033991

• Overview:

  Purified recombinant protein of Mouse cell division cycle 42 (Cdc42), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/12540

• Uniprot URL:

  https://www.uniprot.org/uniprot/P60766

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_009861

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_033991