Ppif (NM_134084) Mouse Recombinant Protein

• Background:

  PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP) . It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F (1) F (0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AW457192; CyP-D; CyP-F; CypD; PPIase

• Gene ID:

  105675

• UniProt:

  Q99KR7

• Accession Number mRNA:

  NM_134084

• Chromosomal Location:

  14 A3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  21.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ppif

• Species:

  Mouse

• Protein ID:

  NP_598845

• Overview:

  Purified recombinant protein of Mouse peptidylprolyl isomerase F (cyclophilin F) (Ppif), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/105675

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q99KR7

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_134084

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_598845