Chmp5 (NM_029814) Mouse Recombinant Protein

• Background:

  Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I, -II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4 (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2210412K09Rik; AW545668

• Gene ID:

  76959

• UniProt:

  Q9D7S9

• Accession Number mRNA:

  NM_029814

• Chromosomal Location:

  4 A5

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  24.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Chmp5

• Species:

  Mouse

• Protein ID:

  NP_084090

• Overview:

  Purified recombinant protein of Mouse charged multivesicular body protein 5 (Chmp5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/76959

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9D7S9

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_029814

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_084090