Nol3 (NM_030152) Mouse Recombinant Protein

• Background:

  Apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly (By similarity) . Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation (By similarity) . Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors (PubMed:16505176) (PubMed:24312627) . Inhibits calcium-mediated cell death by functioning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis (By similarity) . Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation (By similarity) . Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner (By similarity) . Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I (PubMed:24440909) . Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia (PubMed:22082675) . Inhibits too myoblast differentiation through caspase inhibition (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  ARC; B430311C09Rik; MYC; NOP; Nop30

• Gene ID:

  78688

• UniProt:

  Q9D1X0, Q53YU5

• Accession Number mRNA:

  NM_030152

• Chromosomal Location:

  8 53.04 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  24.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Nol3

• Species:

  Mouse

• Protein ID:

  NP_084428

• Overview:

  Purified recombinant protein of Mouse nucleolar protein 3 (apoptosis repressor with CARD domain) (Nol3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/78688

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9D1X0

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_030152

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_084428