Mmachc (NM_025962) Mouse Recombinant Protein

• Background:

  Catalyzes the reductive dealkylation of cyanocobalamin to cob (II) alamin, using FAD or FMN as cofactor and NADPH as cosubstrate. Can also catalyze the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. Under anaerobic conditions cob (I) alamin is the first product; it is highly reactive and is converted to aquocob (II) alamin in the presence of oxygen. Binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  1810037K07Rik; CblC

• Gene ID:

  67096

• UniProt:

  Q9CZD0

• Accession Number mRNA:

  NM_025962

• Chromosomal Location:

  4 D1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  25.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Mmachc

• Species:

  Mouse

• Protein ID:

  NP_080238

• Overview:

  Purified recombinant protein of Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/67096

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9CZD0

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_025962

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_080238