Uchl3 (NM_016723) Mouse Recombinant Protein

• Background:

  Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB (+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.[UniProtKB/Swiss-Prot Function]

• Gene ID:

  50933

• UniProt:

  Q9JKB1, Q8BWQ9

• Accession Number mRNA:

  NM_016723

• Chromosomal Location:

  14 50.9 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  26.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Uchl3

• Species:

  Mouse

• Protein ID:

  NP_057932

• Overview:

  Purified recombinant protein of Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/50933

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9JKB1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016723

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_057932