Otub1 (NM_134150) Mouse Recombinant Protein

• Background:

  Hydrolase that can specifically remove compared to 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1) . Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI850305

• Gene ID:

  107260

• UniProt:

  Q7TQI3

• Accession Number mRNA:

  NM_134150

• Chromosomal Location:

  19 A

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  31.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Otub1

• Species:

  Mouse

• Protein ID:

  NP_598911

• Overview:

  Purified recombinant protein of Mouse OTU domain, ubiquitin aldehyde binding 1 (Otub1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/107260

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q7TQI3

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_134150

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_598911