Spsb1 (NM_029035) Mouse Recombinant Protein

• Background:

  Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity) . Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (By similarity) . Acts as a bridge which links the NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  1110014L01Rik; 4930422J18Rik; AI596360; AI854583; SSB-1; SSB1

• Gene ID:

  74646

• UniProt:

  Q9D5L7

• Accession Number mRNA:

  NM_029035

• Chromosomal Location:

  4 E2

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  30.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Spsb1

• Species:

  Mouse

• Protein ID:

  NP_083311

• Overview:

  Purified recombinant protein of Mouse splA/ryanodine receptor domain and SOCS box containing 1 (Spsb1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/74646

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9D5L7

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_029035

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_083311