Cnot7 (NM_011135) Mouse Recombinant Protein

• Background:

  Has 3'-5' poly (A) exoribonuclease activity for synthetic poly (A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for miRNA-mediated mRNA deadenylation. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AU022737; CAF-1; Caf1; Pop2

• Gene ID:

  18983

• UniProt:

  Q60809, Q543X5

• Accession Number mRNA:

  NM_011135

• Chromosomal Location:

  8 A4

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  32.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Cnot7

• Species:

  Mouse

• Protein ID:

  NP_035265

• Overview:

  Purified recombinant protein of Mouse CCR4-NOT transcription complex, subunit 7 (Cnot7), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/18983

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q60809

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_011135

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_035265