Stx1a (NM_016801) Mouse Recombinant Protein

• Background:

  Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed:17502420, PubMed:28596237, PubMed:28031464) . Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm (PubMed:12101244) . Plays also an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (PubMed:17502420, PubMed:28596237, PubMed:28031464) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  HPC-1

• Gene ID:

  20907

• UniProt:

  O35526, Q497P1, Q5D0A4

• Accession Number mRNA:

  NM_016801

• Chromosomal Location:

  5 G2

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  33.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Stx1a

• Species:

  Mouse

• Protein ID:

  NP_058081

• Overview:

  Purified recombinant protein of Mouse syntaxin 1A (brain) (Stx1a), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/20907

• Uniprot URL:

  https://www.uniprot.org/uniprot/O35526

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016801

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_058081