Atxn3 (NM_001167914) Mouse Recombinant Protein

• Background:

  Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates (By similarity) . Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins (By similarity) . Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension (PubMed:21855799) . Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription (By similarity) . Regulates autophagy via the deubiquitination of 'Lys-402' of BECN1 leading to the stabilization of BECN1 (PubMed:28445460) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2210008M02Rik; AI463012; AI647473; ataxin-3; ATX3; Mjd; MJD1; Sca3

• Gene ID:

  110616

• UniProt:

  Q5M8S1, E9Q717, Q810M8

• Accession Number mRNA:

  NM_001167914

• Chromosomal Location:

  12 E

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  33.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Atxn3

• Species:

  Mouse

• Protein ID:

  NP_001161386

• Overview:

  Purified recombinant protein of Mouse ataxin 3 (Atxn3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/110616

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q5M8S1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001167914

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001161386