Sparc (NM_009242) Mouse Recombinant Protein

• Background:

  Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  BM-40; ON

• Gene ID:

  20692

• UniProt:

  P07214, Q5NCU5

• Accession Number mRNA:

  NM_009242

• Chromosomal Location:

  11 33.04 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  34.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Sparc

• Species:

  Mouse

• Protein ID:

  NP_033268

• Overview:

  Purified recombinant protein of Mouse secreted acidic cysteine rich glycoprotein (Sparc), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/20692

• Uniprot URL:

  https://www.uniprot.org/uniprot/P07214

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_009242

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_033268