Pim2 (BC027376) Mouse Recombinant Protein

• Background:

  Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1) -independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X (L) /BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  DXCch3; Pim-2

• Gene ID:

  18715

• UniProt:

  Q62070

• Accession Number mRNA:

  BC027376

• Chromosomal Location:

  X 3.55 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  34.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Pim2

• Species:

  Mouse

• Overview:

  Purified recombinant protein of Mouse proviral integration site 2 (cDNA clone MGC:37188 IMAGE:4954711), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/18715

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q62070

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/BC027376