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Eif3i (NM_018799) Mouse Recombinant Protein

CAT: 0882-PM47270M5Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0882-PM47270M5Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse eukaryotic translation initiation factor 3, subunit I (Eif3i), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
36kDa; D4Ertd632e; Eif3s2; Trip1
Gene ID
54709
UniProt
Q9QZD9
Accession Number mRNA
NM_018799
Chromosomal Location
4 63.26 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
36.5 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Eif3i
Species
Mouse
Protein ID
NP_061269
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/54709
Uniprot URL
https://www.uniprot.org/uniprot/Q9QZD9
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_018799
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_061269

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